Plasmid Preparation:Article Title: RNA-editing oligonucleotides and uses thereof
Article Snippet: .. For editing experiments, 2 μg of ADAR2/pcDNA3.1 plasmid were transfected into 5×106 HEK293T cells (ATCC) using 25 μL of Lipofectamine 3000 and 24 μL of P3000 (Life Technologies) per 10 cm dish. .. After 4 hours, the culture media was replenished with fresh warmed media (DMEM High Glucose; Life Technologies).
Article Title: RNA-editing oligonucleotides and uses thereof
Article Snippet: .. For editing experiments, 2 μg of ADAR2/pcDNA3.1 plasmid were transfected into 5×106 HEK293T cells (ATCC) using 25 μL of Lipofectamine 3000 and 24 μL of P3000 (Life Technologies) per 10 cm dish. .. After 4 hours, the culture media was replenished with fresh warmed media (DMEM High Glucose; Life Technologies).
Article Title: RNA-editing oligonucleotides and uses thereof
Article Snippet: .. For editing experiments, 2 μg of ADAR2/pcDNA3.1 plasmid were transfected into 5×106 HEK293T cells (ATCC) using 25 L of Lipofectamine 3000 and 24 μL of P3000 (Life Technologies) per 10 cm dish. .. After 4 hours, the culture media was replenished with fresh warmed media (DMEM High Glucose; Life Technologies).
Article Title: RNA-editing oligonucleotides and uses thereof
Article Snippet: .. For editing experiments, 2 g of ADAR2/pcDNA3.1 plasmid were transfected into 5×106 HEK293T cells (ATCC) using 25 μL of Lipofectamine 3000 and 24 μL of P3000 (Life Technologies) per 10 cm dish. .. After 4 hours, the culture media was replenished with fresh warmed media (DMEM High Glucose; Life Technologies).
Article Title: RNA-editing oligonucleotides and uses thereof
Article Snippet: .. For editing experiments, 2 μg of ADAR2/pcDNA3.1 plasmid were transfected into 5×106 HEK293T cells (ATCC) using 25 μL of Lipofectamine 3000 and 24 μL of P3000 (Life Technologies) per 10 cm dish. .. After 4 hours, the culture media was replenished with fresh warmed media (DMEM High Glucose; Life Technologies).
Article Title: RNA-editing oligonucleotides and uses thereof
Article Snippet: .. For editing experiments, 2 μg of ADAR2/pcDNA3.1 plasmid were transfected into 5×106 HEK293T cells (ATCC) using 25 μL of Lipofectamine 3000 and 24 μL of P3000 (Life Technologies) per 10 cm dish. .. After 4 hours, the culture media was replenished with fresh warmed media (DMEM High Glucose; Life Technologies).
Transfection:Article Title: RNA-editing oligonucleotides and uses thereof
Article Snippet: .. For editing experiments, 2 μg of ADAR2/pcDNA3.1 plasmid were transfected into 5×106 HEK293T cells (ATCC) using 25 μL of Lipofectamine 3000 and 24 μL of P3000 (Life Technologies) per 10 cm dish. .. After 4 hours, the culture media was replenished with fresh warmed media (DMEM High Glucose; Life Technologies).
Article Title: RNA-editing oligonucleotides and uses thereof
Article Snippet: .. For editing experiments, 2 μg of ADAR2/pcDNA3.1 plasmid were transfected into 5×106 HEK293T cells (ATCC) using 25 μL of Lipofectamine 3000 and 24 μL of P3000 (Life Technologies) per 10 cm dish. .. After 4 hours, the culture media was replenished with fresh warmed media (DMEM High Glucose; Life Technologies).
Article Title: RNA-editing oligonucleotides and uses thereof
Article Snippet: .. For editing experiments, 2 μg of ADAR2/pcDNA3.1 plasmid were transfected into 5×106 HEK293T cells (ATCC) using 25 L of Lipofectamine 3000 and 24 μL of P3000 (Life Technologies) per 10 cm dish. .. After 4 hours, the culture media was replenished with fresh warmed media (DMEM High Glucose; Life Technologies).
Article Title: RNA-editing oligonucleotides and uses thereof
Article Snippet: .. For editing experiments, 2 g of ADAR2/pcDNA3.1 plasmid were transfected into 5×106 HEK293T cells (ATCC) using 25 μL of Lipofectamine 3000 and 24 μL of P3000 (Life Technologies) per 10 cm dish. .. After 4 hours, the culture media was replenished with fresh warmed media (DMEM High Glucose; Life Technologies).
Article Title: RNA-editing oligonucleotides and uses thereof
Article Snippet: .. For editing experiments, 2 μg of ADAR2/pcDNA3.1 plasmid were transfected into 5×106 HEK293T cells (ATCC) using 25 μL of Lipofectamine 3000 and 24 μL of P3000 (Life Technologies) per 10 cm dish. .. After 4 hours, the culture media was replenished with fresh warmed media (DMEM High Glucose; Life Technologies).
Article Title: RNA-editing oligonucleotides and uses thereof
Article Snippet: .. For editing experiments, 2 μg of ADAR2/pcDNA3.1 plasmid were transfected into 5×106 HEK293T cells (ATCC) using 25 μL of Lipofectamine 3000 and 24 μL of P3000 (Life Technologies) per 10 cm dish. .. After 4 hours, the culture media was replenished with fresh warmed media (DMEM High Glucose; Life Technologies).
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